Exosomes in the Pathogenic Protozoan Trypanosoma Cruzi

The detection of phosphonolipids in the protozoan Trypanosoma cruzi.

2-Aminoethylphosphonate was detected in the acid hydrolysates of the phosphonolipids and the lipopeptidophosphoglycan of Trypanosoma cruzi, the causative agent of Chagas' disease. This finding represents the first evidence of phosphonolipids in a zooflagellate. By comparison, no phosphonolipids were detected in Trypanosama brucei, indicating that phosphonolipids are not a ubiquitous feature of ...

A Genetic Survey of the Pathogenic Parasite Trypanosoma cruzi

In vitro activity of Etanidazole against the protozoan parasite Trypanosoma cruzi.

We investigated the in vitro action of an hydrosoluble 2-nitroimidazole, Etanidazole (EZL), against Trypanosoma cruzi, the etiologic agent of Chagas disease. EZL displayed lethal activity against isolated trypomastigotes as well as amastigotes of T. cruzi (RA strain) growing in Vero cells or J774 macrophages, without affecting host cell viability. Although not completely equivalent to Benznidaz...

The Short Non-Coding Transcriptome of the Protozoan Parasite Trypanosoma cruzi

The pathway for RNA interference is widespread in metazoans and participates in numerous cellular tasks, from gene silencing to chromatin remodeling and protection against retrotransposition. The unicellular eukaryote Trypanosoma cruzi is missing the canonical RNAi pathway and is unable to induce RNAi-related processes. To further understand alternative RNA pathways operating in this organism, ...

Anti-Trypanosoma cruzi activity of extracts from Argentinean Asteraceae species

The anti-Trypanosoma cruzi activity of extracts from 13 Argentinean Asteraceae species was determined. Dichloromethane and methanol extracts of Acmella bellidioides, Aspilia silphioides, Viguiera tuberosa, Calyptocarpus biaristatus, Hyalis argentea, Helenium radiatum, Gaillardia megapotamica, Verbesina subcordata, Gymnocoronis spilanthoides, Viguiera anchusaefolia, Thelesperma megapotamicum, Ze...